Cov kab lus no tau nug cov lus nug: "Zoo li ntawd, dab tsi ntawm cryogenic vial yog qhov no yog tias nws siv tsis tau hauv cov kua nitrogen?"
Tsis yog ib lub lim tiam dhau mus los ntawm qhov uas peb tsis tau hais kom piav qhia qhov tsis txaus ntseeg qhov tsis lees paub uas tshwm sim ntawm txhua nplooj ntawv khoom siv cryovial tsis hais cov chaw tsim khoom, tsis hais txog ntim thiab tsis hais nws cov xov sab hauv cryovial lossis xov sab nraud cryovial.
Cov lus teb yog: Qhov no yog ib qho kev lav phib xaub thiab tsis yog lus nug txog qhov zoo ntawm cryovial.
Cia peb piav.
Zoo li feem ntau cov kav dej ruaj khov, cryovials yog tsim los ntawm qhov kub thiab txias ruaj khov polypropylene.
Lub thickness ntawm polypropylene txiav txim siab qhov kub thiab txias.
Feem ntau 15mL thiab 50mL conical tubes muaj cov phab ntsa nyias uas txwv tsis pub lawv siv rau qhov kub tsis qis dua -86 txog -90 Celsius.
Cov phab ntsa nyias kuj piav qhia tias yog vim li cas 15mL thiab 50mL cov raj conical tsis qhia rau kev sib tw ntawm tus nqi nrawm dua 15,000xg vim tias cov yas tuaj yeem tawg thiab tawg yog tias ua haujlwm dhau qhov pib no.
Cryogenic vials yog tsim los ntawm cov polypropylene thicker uas tso cai rau lawv tuav nyob rau hauv ntau txias txias thiab yuav spun nyob rau hauv ib tug centrifuge ntawm speeds ntau tshaj 25,000xg los yog ntau tshaj.
Qhov teeb meem yog nrog lub hau sealing siv los tiv thaiv lub cryovial.
Rau lub cryovial kom tiv thaiv cov ntaub so ntswg, cell los yog tus kab mob cov qauv nws muaj, lub hau yuav tsum ntsia hlau kom tiav thiab tsim ib tug leakproof foob.
Qhov sib txawv me ntsis yuav tso cai rau evaporation thiab pheej hmoo kis kab mob.
Kev mob siab rau yog tsim los ntawm cov tuam txhab cryovial los tsim cov foob zoo uas yuav suav nrog silicon o-ring thiab / lossis tuab threading rau screwing lub hau tag nrho.
Qhov no yog qhov twg ntawm qhov chaw tsim khoom cryovial tuaj yeem xa tuaj.
Thaum kawg qhov ua tiav lossis tsis ua tiav ntawm cryovial los khaws cov qauv poob rau ntawm tus kws kho mob kom paub meej tias muaj kev foob zoo.
Yog tias lub foob tsis zoo, thiab txawm tias thaum lub hau kaw kom zoo, cov kua nitrogen tuaj yeem nkag mus rau hauv lub cryovial thaum nws submerged hauv cov kua theem kua nitrogen.
Yog tias tus qauv yog thawed sai heev, cov kua nitrogen yuav nthuav dav sai thiab ua rau cov ntsiab lus siab tawg thiab xa cov yas shards rau hauv tes thiab ntsej muag ntawm leej twg tsis muaj hmoo txaus nyob ze.
Yog li ntawd, tsis tshua muaj kev zam, cryovial manufacturers xav kom lawv cov distributors ua siab loj qhia qhov tsis lees paub tsis txhob siv lawv cov cryovials tshwj tsis yog rau cov roj theem ntawm cov kua nitrogen (ib puag ncig -180 txog -186C).
Koj tseem tuaj yeem ua kom nrawm nrawm cov ntsiab lus hauv lub cryovial los ntawm ib nrab submerging nws hauv cov kua theem nitrogen;lawv ruaj txaus thiab yuav tsis tawg.
Xav paub ntau ntxiv txog cov kev phom sij ntawm kev khaws cia cryogenic vials hauv cov kua theem kua nitrogen?
Ntawm no yog ib tsab xov xwm los ntawm UCLA's Center for Laboratory Safety sau txog kev raug mob vim yog qhov tawg ntawm cryovial.
Lub sij hawm xa tuaj: Plaub Hlis-21-2022